Objectives : We have examined the vasoconstriction response to the various extracts of Crotonis Fructus (CF, Seed of Croton tiglium L.), defatted CF (DCF), roasted CF (RCF), and Croton oil in rat isolated thoracic aorta.
Methods : We have examined the thoracic aorta segments obtained from rats immediately after delivery were mounted in organ baths superfused on a polygraph.
Results : We found that the thoracic aorta segments responded to various extracts of CF with a dose-dependent vasoconstriction. In comparison to the 100 mM KCl induced contraction, it was contracted by 150% (water extract of CF), 144.5% (70% ethanol extract of CF), 80.1% (water extract of DCF) and 96.6% (ethanol extract of DCF) after addition of the 3 mg/ml each extract. The thoracic aorta segments were contracted by 118.5% (0.01 mg/ml Croton oil), 133.8% (3 mg/ml RCF for 10min), 123.1% (20min), 117.8% (30min), and 103.9% (40min).
Conclusions : In the conclusion, CF, DCF, and RCF induced dose-dependent contraction in the isolated rat thoracic aorta. And it´s major component, Croton oil, has potent vasoconstriction effect.
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